The Midland Certified Reagent Company, Incorporated
The Company for Quality DNA & RNA Oligos
 
 
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NEWS PAGE

New Nuclease Assay Procedure Developed

 

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In our continuing effort to prepare polynucleotides, both deoxy and ribo, of high molecular weight, our senior scientist, Dr. Richard Case, has developed a new assay procedure for the detection of nucleases in column chromatographic eluates of the enzymes used to prepare these polymers. The assay utilizes the MALDI-TOF mass spectrometer and is suitable for the screening of column eluates.

 

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In brief, a small amount of a column chromatographically eluted fraction, typically 5-10 microliters, is incubated with a solution of a purified, synthetic oligonucleotide in the presence of buffer salts for several hours. A portion of this reaction mixture is then freed of interfering cations and analyzed in the mass spectrometer.

 

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This rapid screening procedure is useful for pooling fractions free of nuclease and has been used to prepare DNA homopolymers of unprecedented size. See for example our current lots of poly dT, which range in size from an average length of 60 to material of greater than 500 nucleotides in length. For further details, contact rcase@oligos.com. RVC



News Article from Local News Paper

 

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Click here to read a local news paper article written about Dr. Case and our company.



Shopping Cart Update

 

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We updated the shopping cart by adding a button which will open another window to list our most common scales of synthesis and their allowable lengths.



WHAT'S NEW IN MODIFICATIONS?

 

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Unique base analogs are released so often that it is difficult for us to keep our list complete! Except for certain patent and licensing agreements, basically if the modification is available commercially, then we can include it within your sequence - or if you have access to a non-commercially-available modified base, contact one of our scientists to see if we can incorporate it within your sequence. Or do you need oligos with multiple modifications within the same sequence? Midland is THE source for all your modified oligonucleotide needs.

 

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Because of possible complications for sequences with multiple modifications, we allow only the most basic and common modifications to be ordered directly through our web site, but we offer literally hundreds more. (You can check out the list by clicking here , and search by type or alphabetically). E-mail us at orders@oligos.com. or call 1-800-247-8766 or 432-694-7950 to discuss your unique research requirements with one of our knowledgeable scientists.



DNA Polymer News

 

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The very first products manufactured by the Midland Certified Reagent Company were polynucleotides, and indeed, these high quality polymers have been a major part of our product line ever since. The key to our success in this field is that we purify our own enzymes, polynucleotide phosphorylase, terminal transferase, and DNA polymerase. We have developed an assay procedure for monitoring column chromatography eluates for nuclease, enabling us to pool enzyme fractions with very low levels of these competing, degradative activities.

 

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After the enzymatic synthesis, our polymers are deproteinized and then dialyzed exhaustively to convert to the desired salt form and to remove traces of unwanted counterions. Our polynucleotides are characterized for molecular weight, ultraviolet absorbance, and absorbancy per unit weight. These data are provided on the Certificate of Analysis for each lot.

 

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We continue to be a manufacturing source for DNA and RNA polymers. We do not purchase polymers from other manufacturers for resale. Thus when you need technical assistance with polynucleotides, the people at Midland have firsthand knowledge of the preparative methods, and we can answer your questions based on more than two decades of manufacturing experience.



MOLECULAR BIOLOGY

 

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We are presently reorganizing the molecular biology portion of our business. During this transition period, we will continue to make the very high quality oligonucleotides that we use for gene construction. We are redirecting our focus to the far greater numbers of oligonucleotides that will be required for genome synthesis. Our objective will be to make the very large numbers of oligos of extraordinarily high quality that will be needed to build synthetic genomes. Meanwhile, some of the other portions of our service have been interrupted or discontinued. We apologize for any inconvenience this may cause.

 

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Many of our customers now make their genes themselves, using our very high quality oligonucleotides, typically 40-mers, to make constructs of 1 to 1.5 kb. The quality of the synthetic oligos is the critical factor in these constructs. If a single deletion within an oligo is incorporated into the construct, the entire downstream sequence will be read out of frame, and hence will be useless until a mutagenesis is performed to correct the error. But this mutagenesis reaction is costly and causes delays in the accurate building of genes, hence our emphasis on the quality of the initial input oligos.

 

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Please contact our company president, Dr. Lynn Myers at lmyers@oligos.com, to convey your needs and requirements, and we will try to be as responsive as possible.


We would greatly appreciate any comments you have regarding our website. Please e-mail any suggestions you may have to walter@oligos.com


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